Literature summary extracted from
Triki, D.; Billot, T.; Visseaux, B.; Descamps, D.; Flatters, D.; Camproux, A.C.; Regad, L.
Exploration of the effect of sequence variations located inside the binding pocket of HIV-1 and HIV-2 proteases (2018), Sci. Rep., 8, 5789 .
Application
EC Number |
Application |
Comment |
Organism |
---|
3.4.23.16 |
medicine |
the HIV proteases are effective therapeutic targets for treating HIV infection because of the essential role in hydrolysing the viral Gag and Gag-Pol precursor polyprotein during infectious viral particle maturation |
Human immunodeficiency virus 1 |
3.4.23.47 |
medicine |
the HIV proteases are effective therapeutic targets for treating HIV infection because of the essential role in hydrolysing the viral Gag and Gag-Pol precursor polyprotein during infectious viral particle maturation |
Human immunodeficiency virus 2 |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.4.23.16 |
HIV protease PR1, sequence comparisons with PR2 |
Human immunodeficiency virus 1 |
3.4.23.47 |
HIV protease PR2, sequence comparisons with PR1 |
Human immunodeficiency virus 2 |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.4.23.16 |
C67A |
naturally occuring mutation |
Human immunodeficiency virus 1 |
3.4.23.16 |
C95A |
naturally occuring mutation |
Human immunodeficiency virus 1 |
3.4.23.16 |
L33I |
naturally occuring mutation |
Human immunodeficiency virus 1 |
3.4.23.16 |
L63I |
naturally occuring mutation |
Human immunodeficiency virus 1 |
3.4.23.16 |
L76M |
site-directed mutagenesis, mutant enzyme structure modelling |
Human immunodeficiency virus 1 |
3.4.23.16 |
additional information |
modelling of PR1 mutant structures containing V32I and L76M substitutions reveals a cooperative mechanism leading to structural deformation of flap-residue 45 that can modify PR2 flexibility |
Human immunodeficiency virus 1 |
3.4.23.16 |
Q7K |
naturally occuring mutation |
Human immunodeficiency virus 1 |
3.4.23.16 |
V32I |
site-directed mutagenesis, mutant enzyme structure modelling |
Human immunodeficiency virus 1 |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.4.23.16 |
additional information |
comparison of the HIV-1 protease (PR1) and HIV-2 protease (PR2) binding pockets extracted from structures complexed with 12 ligands, overview. Structural comparison of PR1 and PR2 pockets highlight structural changes induced by their sequence variations. PR2 pockets are more hydrophobic with more oxygen atoms and fewer nitrogen atoms than PR1 pockets. Modelling of PR1 mutant structures containing V32I and L76M substitutions reveals a cooperative mechanism leading to structural deformation of flap-residue 45 that can modify PR2 flexibility. Substitutions in the PR1 and PR2 pockets can modify PI binding and flap flexibility, which might underlie PR2 resistance against PIs |
Human immunodeficiency virus 1 |
|
3.4.23.47 |
additional information |
HIV-2 protease (PR2) is naturally resistant to most FDA approved HIV-1 protease inhibitors (PIs), a major antiretroviral class. Comparison of the HIV-1 protease (PR1) and HIV-2 protease (PR2) binding pockets extracted from structures complexed with 12 ligands, overview. Structural comparison of PR1 and PR2 pockets highlight structural changes induced by their sequence variations. PR2 pockets are more hydrophobic with more oxygen atoms and fewer nitrogen atoms than PR1 pockets. Specifically, substitutions at residues 31, 46, and 82 induce structural changes in their main-chain atoms that can affect PI binding in PR2. Substitutions in the PR1 and PR2 pockets can modify PI binding and flap flexibility, which might underlie PR2 resistance against PIs |
Human immunodeficiency virus 2 |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.23.16 |
Human immunodeficiency virus 1 |
P04587 |
Gag-Pol polyprotein; HIV-1 |
- |
3.4.23.47 |
Human immunodeficiency virus 2 |
P04584 |
Gag-Pol polyprotein; HIV-2 |
- |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.4.23.16 |
HIV-2 protease |
- |
Human immunodeficiency virus 1 |
3.4.23.16 |
PR2 |
- |
Human immunodeficiency virus 1 |
3.4.23.47 |
HIV-2 protease |
- |
Human immunodeficiency virus 2 |
3.4.23.47 |
PR2 |
- |
Human immunodeficiency virus 2 |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
3.4.23.16 |
evolution |
HIV proteases PR1 and PR2 share only approximately 50% of sequence identity but they exhibit a similar global fold |
Human immunodeficiency virus 1 |
3.4.23.16 |
additional information |
comparison of the HIV-1 protease and HIV-2 protease binding pockets extracted from structures complexed with 12 ligands, overview |
Human immunodeficiency virus 1 |
3.4.23.47 |
evolution |
HIV proteases PR1 and PR2 share only approximately 50% of sequence identity but they exhibit a similar global fold |
Human immunodeficiency virus 2 |
3.4.23.47 |
additional information |
comparison of the HIV-1 protease and HIV-2 protease binding pockets extracted from structures complexed with 12 ligands, overview |
Human immunodeficiency virus 2 |